Bacteriology Essays

Microbiology Laboratory Report

Objectives Aim of this experiment is to differentiate between the two major categories of bacteria , gram positive and gram negative. Through this experiment, gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result, Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour , while Staphylococcus aureus ( S.aureus ) is a positive gram bacteria which stain purple colour. Materials Bacteria : Escherichia coli , Staphylococcus aureus Crystal Violet Gram’s iodine…

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Bacteria Lab Report

The most common way of alleviating the specific symptoms arrived from diseases is to ingest antimicrobial drugs. Chemotherapeutical antimicrobial agents are chemical compounds intended to inhibit or kill rapidly dividing microorganisms. In order to derive an antimicrobial drug, different chemical compounds must be synthetically formed. Among these specific chemical compounds is a compound famously known as the antibiotic. The antibiotic is classified as an antimicrobial drug because it’s a chemical that is produced by either a bacterium or fungus, which…

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Identifying Bacteria

Bacteria identification is accomplished in a number of ways. Two common tools microbiologists use to identify unknown bacteria include dichotomous key and biochemical tests. The dichotomous key is useful when a microbiologist only needs to know which group an unknown microbe belongs to on a general level. When a microbiologist needs to identify a specific bacterium, biochemical tests are used. PART ONE: GENERAL BACTERIA IDENTIFICATION Review the dichotomous key in Figure A, the bacterial shapes in Figure B, and the…

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2d Gel Electrophoresis

Two-dimensional (2-D) gel electrophoresis will be performed according to the method of O’Farrell. Proteins present in the pellet will be resolved on two-dimensional gels using the products and protocols of Amersham Pharmacia Biotech. In the first-dimension, proteins will be resolved by isoelectric focusing on a precast Immobiline DryStrip with a linear pH gradient. This will be followed by electrophoresis using sodium dodecyl sulfate polyacrylamide gel on 12. 5% acrylamide gel. For analytical 2-D gel electrophoresis, 100 ?g of sample protein…

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